Single-cell RNA-Seq Tools

MuSiC utilizes cell-type specific gene expression from single-cell RNA sequencing (RNA-seq) data to characterize cell type compositions from bulk RNA-seq data in complex tissues. By appropriate weighting of genes showing cross-subject and cross-cell consistency, MuSiC enables the transfer of cell type-specific gene expression information from one dataset to another.

Drop-seq is a high-throughput, droplet-based single-cell RNA sequencing (scRNA-seq) technology that allows for genome-wide expression profiling of thousands of individual cells simultaneously. It works by encapsulating single cells and barcoded microbeads into nanoliter-sized aqueous droplets in oil using a microfluidic device.

Pipeline for estimating molecular count matrices for droplet-based single-cell RNA-seq measurements. I